Random Amplification of Polymorphic DNA Technology Service

Random Amplification of Polymorphic DNA Technology Service

The random amplified polymorphic DNA (RAPD) technique is based on PCR technology. It uses a series of oligonucleotide single strands with different randomly arranged base sequences as primers to separate the genomic DNA studied by PCR amplification polyacrylamide or agarose electrophoresis, and detect the polymorphism of amplified DNA fragments by staining or autoradiography. The polymorphism of DNA fragments of these amplified products reflects the DNA polymorphism of the corresponding regions of the genome. RAPD can detect the polymorphism of the whole plant genomic DNA. Lifeasible provides professional, accurate, and fast RAPD technology services to customers worldwide.

Schematic diagram of RAPD reaction for 2 loci.Figure 1. Schematic diagram of RAPD reaction for 2 loci. (Bardakci F, et al. 2001)

  • Applications
  • Due to the simplicity and low cost of the RAPD technique, it is widely used in many fields of biology.

    • Genetic mapping
    • Developing genetic markers linked to a trait in question
    • Population and evolutionary genetics
    • Plant breeding
  • What We Offer
    • Customers can contact us directly on the website to request a RAPD technical service order sent to our company email. If you have any questions in this process, you can contact us at any time for details, including the specific requirements and service contents.
    • After the end of the experiment, the company will immediately send the results to the customer in time, and at the same time deal with the relevant materials according to the customer's requirements.
    • After the experiment, you can receive the primer sequence, PCR reaction system, and electrophoresis pictures, including specific experimental methods, steps, reagents, instruments, and related analysis data, etc., this series of experimental results will be submitted to you.
  • Service Flow
  • Service Flow

  • Why Choose Lifeasible
  • Lifeasible provides RAPD technology services for customers around the world, making use of its unique way of detecting DNA polymorphism and its fast and simple characteristics to provide you with a full range of services for plant genome polymorphism research.

    • The use of lower annealing temperature not only ensures the stable pairing of short nucleotide primers and template but also allows proper mismatch, which expands the randomness of primer pairing.
    • More professional and comprehensive system analysis, make the best use of the experimental results.
    • A set of primers has been synthesized for you, which can be used for the analysis of different biological genomes to save you cost.
    • We provide the most sensitive and convenient RAPD detection using fluorescent dyes or isotope markers to greatly improve the speed of analysis and make the results more quickly fed back to you.
    • Analysis requires only a small amount of plant DNA sample.
    • The use of automated amplification analysis reduces the tedious process and allows you to get results faster.
  • How to Place an Order

How to Place an Order

Lifeasible offers complete, professional RAPD technology service, as well as customized experimental protocols based on your project requirements and plant sample characteristics. For more information, please contact Lifeasible.

Reference:

  1. Bardakci F. "Random amplified polymorphic DNA (RAPD) markers." Turkish Journal of Biology, 2001, 25 (2): 185-196.
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